《植物生理学报》 2014, 50(2): 229-232

一种便于丝状真菌显微观察的培养方法

孙建广¹, 周欣¹, 肖佳雷², 尹静^1,*, 詹亚光¹, 战妍¹, 姜丽超¹, 巩媛¹

¹东北林业大学生命科学学院, 哈尔滨150040; ²黑龙江省农业科学院耕作栽培研究所, 哈尔滨150086

通信作者：尹静；E-mail: yinjing20135@163.com；Tel: 0451-82191752

摘　要：

在真菌培养过程中, 对其个体形态与群体(菌落)形态进行实时观察与鉴定是很必要的。本文利用半培养基培养法结合显微操作技术, 对丝状真菌个体与群体进行形态学观察。结果表明, 该方法无需染色与制片, 不破坏菌丝正常生长状态, 可实时进行形态学检测, 对多个菌种在自然生长状态下的菌丝与菌落特征进行观察, 操作简单、方便、快捷, 从而降低了成本及工作量。

关键词：丝状真菌; 形态观察; 鉴定

收稿：2013-09-16   修定：2014-01-20

资助：国家基础科学人才培养基金(J1210053)、国家自然科学基金(31101171)、东北林业大学高等学校教学改革及细胞生物学重点课程建设项目。

A Convenient Culture Method for Observing Filamentous Fungi

SUN Jian-Guang¹, ZHOU Xin¹, XIAO Jia-Lei², YIN Jing^1,*, ZHAN Ya-Guang¹, ZHAN Yan¹, JIANG Li-Chao¹, GONG Yuan¹

¹College of Life Sciences, Northeast Forestry University, Harbin 150040, China; ²Farming and Cultivation Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

Corresponding author: YIN Jing; E-mail: yinjing20135@163.com; Tel: 0451-82191752

Abstract:

It is important to observe and identify the morphology of individual form and group (colony) during the cultivation of fungi. In this study, half medium combined with micromanipulation technique was used to observe the morphological changes in individual and group of filamentous fungi. The results showed that this novel method could be used to make real-time measurements of the morphology of filamentous fungi without dyeing and slicing, and does not affect the normal growth of mycelia. In addition, it can be applied to many strains for observing the mycelium undergoing natural growth and the colony characteristics simultaneously. Overall, in this experiment, a simple and rapid method is designed for the observation of individual and colony morphology of filamentous fungi, which needs a low cost and workload.

Key words: filamentous fungi; morphological observation; identification